MINK1 (Human) Recombinant Protein
产品名称: MINK1 (Human) Recombinant Protein
英文名称: MINK1 (Human) Recombinant Protein
产品编号: P5730
产品价格: 0
产品产地: 台湾
品牌商标: Abnova
更新时间: null
使用范围: null
亚诺法生技股份有限公司(Abnova)
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- Specification
- Product Description:
- Human MINK1 (NP_056531.1, 1 a.a. - 314 a.a.) partial recombinant protein with GST tag expressed in Baculovirus infected Sf21 cells.
- Theoretical MW (kDa):
- 63
- Form:
- Liquid
- Preparation Method:
- Baculovirus infected insect cell (Sf50) expression system
- Purification:
- Glutathione sepharose chromatography and anion exchange chromatography
- Purity:
- 94 % by SDS-PAGE/CBB staining.
- Activity:
- The activity was measured by off-chip mobility shift assay. The enzyme was incubated with fluorecence-labeled substrate and Mg (or Mn)/ATP. The phosphorylated and unphosphorylated substrates were separated and detected by LabChip™3000.
Substrate : Modified Erktide. ATP: 100 µM.
- Storage Buffer:
- In 50 mM Tris-HCl, 150 mM NaCl, pH 7.5 (0.05% Brij35, 1 mM DTT, 10% glycerol)
- Storage Instruction:
- Store at -80°C.
Aliquot to avoid repeated freezing and thawing.
- Quality Control Testing:
- Loading 1 ug protein in SDS-PAGE
- Note:
- Result of activity analysis
-
- Application Image
- Functional Study
- SDS-PAGE
- Entrez GeneID:
- 50488
- Protein Accession#:
- NP_056531.1
- Gene Name:
- MINK1
- Gene Alias:
- B55,MAP4K6,MGC21111,MINK,YSK2,ZC3,hMINK,hMINKbeta
- Gene Description:
- misshapen-like kinase 1 (zebrafish)
- Omim ID:
- 609426
- Gene Ontology:
- Hyperlink
- Gene Summary:
- This gene encodes a serine/threonine kinase belonging to the germinal center kinase (GCK) family. The protein is structurally similar to the kinases that are related to NIK and may belong to a distinct subfamily of NIK-related kinases within the GCK family. Studies of the mouse homolog indicate an up-regulation of expression in the course of postnatal mouse cerebral development and activation of the cJun N-terminal kinase (JNK) and the p38 pathways. Alternative splicing occurs at this locus and four transcript variants encoding distinct isoforms have been identified. [provided by RefSeq
- Other Designations:
- GCK family kinase MINK,misshapen/NIK-related kinase,serine/threonine protein kinase